P4HAS as modifiers of the IGFR pathway and methods of use
AssigneeExelixis, Inc.
InventorsLori S. Friedman, Helen Francis-Lang, Annette L. Parks, Kenneth James Shaw, Timothy S. Heuer, Lynn Margaret Bjerke
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Abstract
Human P4HA genes are identified as modulators of the IGFR pathway, and thus are therapeutic targets for disorders associated with defective IGFR function. Methods for identifying modulators of IGFR, comprising screening for agents that modulate the activity of P4HA are provided.
Claims25
A method of identifying a candidate IGFR pathway modulating agent, said method comprising the steps of:
- (a) providing an assay system comprising a P4HA polypeptide or nucleic acid;
- (b) contacting the assay system with a test agent under conditions whereby, but for the presence of the test agent, the system provides a reference activity;
- and
- (c) detecting a test agent-biased activity of the assay system, wherein a difference between the test agent-biased activity and the reference activity identifies the test agent as a candidate IGFR pathway modulating agent.
A method for modulating a IGFR pathway of a cell comprising contacting a cell defective in IGFR function with a candidate modulator that specifically binds to a P4HA polypeptide, whereby IGFR function is restored.
A method of modulating IGFR pathway in a mammalian cell comprising contacting the cell with an agent that specifically binds a P4HA polypeptide or nucleic acid.
A method for diagnosing a disease in a patient comprising:
- obtaining a biological sample from the patient;
- contacting the sample with a probe for P4HA expression;
- comparing results from step (b) with a control;
- determining whether step (c) indicates a likelihood of disease.
Dependencies are inferred from the claim text.