Diagnostic kit for detecting pulmonary and extra pulmonary tuberculosis
AssigneesDepartment of Biotechnology, Madhav Institute of Technology and Science
InventorsPrakash Singh Bisen, Ram Pramod Tiwari
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Abstract
A diagnostic kit for detecting pulmonary and extra pulmonary tuberculosis comprising a test card “TB Screen” coated with a hydrophobic material, antigen suspension, positive and negative control.
Claims15
A diagnostic kit for detecting pulmonary and extra pulmonary tuberculosis, comprising a test card coated with a hydrophobic material, mixing sticks, a glycolipid from a Mycobacterium tuberculosis H37RV antigen suspension intercalated or coupled with a liposome surface, a positive control comprising an antibody that binds to said glycolipid from Mycobacterium tuberculosis, and a negative control comprising serum antibodies from a subject not previously exposed to Mycobacterium tuberculosis.
A method for testing an individual for tuberculosis comprising the steps of applying a positive control, a negative control and a sample to a hydrophobic material, wherein said positive control is an antibody that binds to a glycolipid from Mycobacterium tuberculosis, and wherein said negative control is a serum antibody from a subject not previously exposed to Mycobacterium tuberculosis;
- adding an antigen suspension to said positive, said negative and said sample;
- and interpreting a result, wherein clumping of a specific antigen in the suspension and an antibody in the positive control and a test sample from the individual is prognostic for an active tuberculosis infection, and wherein the antigen is a glycolipid antigen from Mycobacterium tuberculosis H37Rv (ATCC-27294).
Dependencies are inferred from the claim text.