Use of ID4 for diagnosis and treatment of cancer
AssigneeJohn Wayne Cancer Institute
InventorsDave S. B. Hoon, Naoyuki Umetani
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Abstract
The invention relates to a method of determining whether a human subject is suffering from or at risk for developing pancreatic cancer by determining the methylation level of an ID4 gene promoter or the expression level of an ID4 gene in a biological sample from a human subject. Also disclosed are a method of analyzing the methylation level of an ID4 gene promoter or the expression level of an ID4 gene in a pancreatic cancer cell, and a method of inhibiting the methylation of an ID4 gene promoter or enhancing the expression of an ID4 gene by contacting a pancreatic cancer cell with a compound that decreases the methylation level of an ID4 gene promoter or increases the expression level of an ID4 gene in the cell.
Claims20
A method of determining whether a human subject is suffering from or at risk for developing pancreatic cancer, comprising:
- obtaining a pancreatic tumor tissue sample from the human subject;
- measuring a methylation level of an ID4 (inhibitor of DNA binding 4) gene promoter or an expression level of an ID4 gene;
- and
- determining that the human subject is likely to be suffering from or at risk for developing pancreatic cancer when the methylation level of the ID4 gene promoter is higher than a control methylation level or the expression level of an ID4 gene in the sample is lower than a control expression level.
A method for diagnosing a migratory or invasive phenotype of a pancreatic tumor in a human subject, comprising:
- obtaining a pancreatic tumor tissue sample from the human subject;
- measuring a methylation level of an ID4 (inhibitor of DNA binding 4) gene promoter or an expression level of an ID4 gene;
- and
- determining that the pancreatic tumor is likely to be migratory or invasive when the methylation level of the ID4 gene promoter is higher than a control methylation level or the expression level of an ID4 gene in the sample is lower than a control expression level.
A method for determining a migratory or invasive pancreatic cancer cell phenotype, comprising:
- obtaining a pancreatic cancer cell from a pancreatic cell culture or a pancreatic tumor tissue from a subject having pancreatic cancer;
- measuring a methylation level of an ID4 (inhibitor of DNA binding 4) gene promoter or an expression level of an ID4 gene;
- and
- determining that the pancreatic cancer cell is likely to be migratory or invasive when the methylation level of the ID4 gene promoter is higher than a control methylation level or the expression level of an ID4 gene in the sample is lower than a control expression level.
Dependencies are inferred from the claim text.