A method for identifying a dairy cattle or an embryo thereof as having an increased likelihood of having a phenotype that provides increased or decreased milk yield, increased milk fat yield, increased or decreased milk fat percentage, decreased milk protein yield, increased or decreased milk protein percentage, increased or decreased productive life (PL), increased or decreased somatic cell score (SCS), or increased or decreased daughter pregnancy rate (DPR), said method comprising haplotyping said cattle or embryo thereof by:
- (i) detecting in the proteinase inhibitor (PI) gene of said dairy cattle or embryo thereof the presence of haplotype 1, wherein haplotype 1 comprises the nucleotides A, C, G, C and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 1 is indicative of an increased likelihood of having an increased milk protein percentage, increased PL, increased SCS, or increased DPR phenotype;
- or
- (ii) detecting in the PI gene of said dairy cattle or embryo thereof the presence of haplotype 2, wherein haplotype 2 comprises the nucleotides G, T, T, G and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 2 is indicative of an increased likelihood of having a decreased milk yield or decreased protein yield phenotype;
- or
- (iii) detecting in the PI gene of said dairy cattle or embryo thereof the presence of haplotype 3, wherein haplotype 3 comprises the nucleotides G, C, G, G and T at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 3 is indicative of an increased likelihood of having an increased milk fat yield phenotype;
- or
- (iv) detecting in the PI gene of said dairy cattle or embryo thereof the presence of haplotype 4, wherein haplotype 4 comprises the nucleotides G, T, T, G and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 4 is indicative of an increased likelihood of having an increased milk fat percentage, decreased milk protein percentage, decreased PL, decreased SCS or decreased DPR phenotype;
- or
- (v) detecting in the PI gene of said dairy cattle or embryo thereof the presence of haplotype 5, wherein haplotype 5 comprises the nucleotides G, C, G, G and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 5 is indicative of an increased likelihood of having an increased milk yield, decreased milk fat percentage, or decreased milk protein percentage phenotype;
- or
- (vi) detecting in the PI gene of said dairy cattle or embryo thereof the presence of haplotype 6, wherein haplotype 6 comprises the nucleotides A, C, G, C and C at the positions corresponding to positions 164, 269, 284, 407 and 989, respectively, of SEQ ID NO:
- 1, wherein the presence of haplotype 6 is indicative of an increased likelihood of having a decreased PL, decreased SCS, or decreased DPR.