Method of detecting Haemophilus influenzae type b, primer set and kit for the use in the method

Abstract

The present invention provides a method of detecting Haemophilus influenzae, which enables accurate and rapid detection of H. influenzae, a primer set for detecting H. influenzae, and a kit for detecting H. influenzae. Nucleic acid amplification is carried out using the DNA of H. influenzae as a template, and also using the LAMP primers as shown in SEQ ID NOS: 1 to 5 given as examples of the present invention. Thus, the presence or absence of the amplified product is detected. When primers having sequences that are complementary to the sequences as shown in SEQ ID NOS: 1 to 5 are used, such primers are excellent in terms of detection sensitivity and promptness of detection, as well as specificity. In addition, as another example of the present invention, nucleic acid amplification is carried out using LAMP primers as shown in SEQ ID NOS: 43 to 47, and the presence or absence of the amplified product is detected. Thereby, H. influenzae Type b can be distinguished from other capsular serotype and non-encapsulated type H. influenzae, and it can be detected rapidly, simply, and accurately.

Dependencies are inferred from the claim text.