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US 7,888,006 B2Granted
Method for isolating DNA from biological samples
AssigneeQiagen GmbH
InventorChristian Lenz
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Abstract
The present invention relates to an improved process for isolating DNA from biological samples, particularly from human whole blood.
Claims54
A process for isolating DNA from a biological sample, comprising
- (a) mixing a biological sample with a lysing reagent,
- (b) separating the DNA-containing cell components from the other cell components in the mixture,
- (c) resuspending the DNA-containing cell components in a saline solution which contains a chaotropic salt,
- (d) removing contaminating ingredients from the resulting suspension by chemical, thermal and/or enzymatic treatment,
- (e) precipitating the DNA and retaining the precipitate,
- (f) washing the DNA precipitate,
- (g) retaining and drying the washed DNA, and
- (h) optionally resuspending the DNA in a low salt solution, wherein the isolation process is carried out in one reaction vessel and wherein the lysing reagent comprises, at least one salt of a mono- or divalent cation and one or more complexing agents;
- wherein the lysing reagent is not susceptible to microbial growth.
6 more elements
Dependencies are inferred from the claim text.