Method of manufacturing plastic substrate using plasma process and plastic substrate manufactured using the method

A method of manufacturing a bio-chip substrate, the method comprising:

• (a) preparing a plastic substrate having an Atomic Force Microscopic (AFM) surface roughness of Ra<3 nm or Rq<4 nm before being treated, wherein Ra is an average roughness and Rq is a root-mean-square roughness;
• (b) treating the plastic substrate with argon plasma to form a radical on the surface of the plastic substrate;
• and then
• (c) treating the plastic substrate with a surface-modifying monomer, wherein the radical is reacted with the surface-modifying monomer to form a compound on the surface of the plastic substrate,
• wherein the surface-modifying monomer is one of allyl-glycidylether having an epoxide group and a carbon-carbon double bond, and glycidylmethacrylate having an epoxide group and a carbon-carbon double bond, the plastic substrate being surface-modified with the epoxide group;
• one of hexanal, octanal, and nonanal each of which has an aldehyde group and a boiling point of 80° C. or higher, the plastic substrate being surface-modified with the aldehyde group;
• or one of allylamine having a primary amine group and a carbon-carbon double bond, and 3-methoxypropylamine which is an ether compound having a primary amine group, the plastic substrate being surface-modified with the amine group;
• and
• (d) preparing the plastic substrate treated in (c) as the bio-chip substrate to be used with fluorescent material-labeled biomolecules to be detected by fluorescent measurement.