Optimized DNA and protein sequence of an antibody to improve quality and yield of bacterially expressed antibody fusion proteins
AssigneeTopoTarget Germany AG
InventorsWinfried S. Wels, Benjamin Daelken, Sylvia E. Schwarz
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Abstract
Object matter of the invention is an optimized DNA sequence encoding the scFv(FRP5) antibody fragment. This novel sequence prevents the generation of the undesired by-product in the context of an scFv(FRP5)-ETA fusion protein, and possibly also other bacterially expressed scFv(FRP5)-containing fusion proteins. The DNA sequence of the scFv(FRP5) domain of scFv(FRP5)-ETA was modified by exchanging a distinct codon, thereby preventing an otherwise possible internal start of protein translation.
Claims22
An isolated polypeptide comprising a first amino acid sequence which comprises the amino acids 2-120 of SEQ ID NO:11, and a second amino acid sequence which comprises the amino acids 136-242 of SEQ ID NO:11, wherein said first amino acid sequence and said second amino acid sequence are linked by a peptide spacer group.
An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:11, SEQ ID NO:1 or SEQ ID NO:12.
Dependencies are inferred from the claim text.