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US 7,887,752 B2Granted
Chemical reaction monitor
AssigneeIllumina, Inc.
InventorsDavid Louis Heiner, Steven P. Fambro, Theo Kotseroglou, Michal Lebl
Life of this patent
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Abstract
Disclosed herein are systems for monitoring chemical reactions. The systems can comprise a lighting device, a camera device for obtaining an image of the chemical reaction mixtures and an analyzer program to process data obtained from the image. Also disclosed are methods of monitoring the progress of chemical reactions using the these systems.
Claims41
A chemical reaction monitoring system for parallel monitoring of a plurality of chemical reactions, said system comprising:
- a plurality of wells, each well of said plurality of wells comprising a reagent for a chemical reaction;
- a lighting device for illuminating the plurality of wells;
- a camera device configured to obtain an image of the plurality of wells
- an analyzer program for determining a specific value corresponding to the extent of chemical reaction within each well at the time the image was obtained;
- an analyzer program for determining whether a difference between a value expected if the chemical reaction is successful and said specific value indicates failure of the chemical reaction within a well;
- and
- a dispensing device configured to discontinue reagent delivery to one or more wells where failure is indicated while maintaining reagent delivery to wells where failure is not indicated, wherein said analyzer program includes an instruction to resume reagent delivery to said one or more wells if one or more criteria are met.
4 more elements
A chemical synthesis system comprising:
- (a) a sample holder placed to support a plurality of wells;
- (b) a liquid dispenser placed to dispense a liquid to said plurality of wells;
- (c) a liquid removal device placed to remove said liquid from said plurality of wells;
- (d) a lighting device for illuminating said plurality of wells;
- (e) a camera device configured to an obtain image of said plurality of wells;
- and
- (f) a computer system configured to:
- (i) determine a specific value corresponding to the extent of chemical reaction within each well at the time the image was obtained;
- (ii) determine whether a difference between a value expected if the chemical reaction is successful and said specific value indicates failure of the chemical reaction within a well;
- and
- (iii) signal said liquid dispenser to discontinue reagent delivery to one or more wells where failure is indicated while maintaining reagent delivery to wells where failure is not indicated, wherein said computer system comprises an analyzer program including an instruction to resume reagent delivery to said one or more wells if one or more criteria are met.
8 more elements
A system for monitoring polymer synthesis, said system comprising:
- a plurality of wells, each well of said plurality of wells providing a location for the synthesis of a polymer from a plurality of reaction components;
- an analyzer program for determining a specific value corresponding to the extent of incorporation of a reaction component into a growing polymer within each well;
- an analyzer program for determining whether a difference between a value expected if incorporation of the reaction component into the growing polymer is successful and said specific value indicates failure of the polymer synthesis within a well;
- and
- a dispensing device configured to discontinue reagent delivery to one or more wells where failure is indicated while maintaining reagent delivery to wells where failure is not indicated, wherein said analyzer program includes an instruction to resume reagent delivery to said one or more wells if one or more criteria are met.
2 more elements
Dependencies are inferred from the claim text.