Method for analyzing haemoglobin by capillary electrophoresis, a kit for capillary electrophoresis, and use of a flow inhibitor in said method
AssigneeSebia
InventorsFrédéric Robert, Denis Simonin, Jean-Baptiste Clement
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Abstract
The invention concerns a method for free solution capillary electrophoresis at an alkaline pH to analyze samples comprising haemoglobin, in which the sample is passed through a capillary containing an analysis buffer, comprising at least one step in which the sample is introduced into a capillary tube containing a solution of analysis buffer, characterized in that the buffer is of the zwitterionic type and in that it is associated with at least one flow inhibitor. It also concerns the use of CE flow inhibitors associated with at least one zwitterionic buffer, and a kit for analyzing haemoglobin by capillary electrophoresis.
Claims24
A free solution capillary electrophoresis method for separating and resolving haemoglobins in a biological sample by capillary electrophoresis, said biological sample being a blood sample, said method comprising:
- introducing said biological sample into a capillary tube comprising a solution of an analysis buffer, wherein said analysis buffer is of the zwitterionic type and at least one flow inhibitor, and
- electrophoresing said biological sample in said analysis buffer and at least one said flow inhibitor, whereby said haemoglobins are separated by migration, and wherein said haemoglobins are a haemoglobin HbA2 and an HbC, an HbD, an HbE, an HbS, an HbF or an HbA haemoglobin.
A free solution capillary electrophoresis method for separating and resolving haemoglobins in a biological sample by capillary electrophoresis, said biological sample being a blood sample, said sample being selected from the group consisting of normal blood, abnormal blood, washed blood, decanted blood, centrifuged blood, whole blood and haemolyzed blood, said method comprising:
- introducing said biological sample into a capillary tube comprising a solution of an analysis buffer, wherein said analysis buffer is of the zwitterionic type and at least one flow inhibitor, and
- electrophoresing said biological sample in said analysis buffer and at least one said flow inhibitor, whereby said haemoglobins are separated by migration.
A free solution capillary electrophoresis method for separating and resolving haemoglobins in a biological sample by capillary electrophoresis, said biological sample being a blood sample, said method comprising:
- introducing said biological sample into a capillary tube comprising a solution of an analysis buffer, wherein said analysis buffer is of the zwittenionic type and at least one flow inhibitor, said flow inhibitor being an aliphatic or a cyclic diamine or a polyamine type, and
- electrophoresing said biological sample in said analysis buffer and at least one said flow inhibitor, whereby said haemoglobins are separated by migration.
Dependencies are inferred from the claim text.