Ketoreductase polypeptides for the production of azetidinone
AssigneeCodexis, Inc.
InventorsOnorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
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Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Claims27
An engineered ketoreductase polypeptide capable of converting the substrate, methyl-2-benzamidomethyl-3-oxobutyrate, to the product, 2S,3R-methyl-2-benzamidomethyl-3-hydroxybutyrate, with a percent stereomeric excess of at least 60%, which comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 4 and in which the residue corresponding to X94 is threonine, the residue corresponding to X199 is alanine, histidine, or asparagine;
- and the residue corresponding to X202 is valine or leucine.
Dependencies are inferred from the claim text.