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US 7,880,057 B2Granted
Precise breeding—backbone integration markers
AssigneeJ.R. Simplot Company
InventorsCaius Rommens, Kathy M. M. Swords
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AssigneeJ.R. Simplot Company
InventorsCaius Rommens, Kathy M. M. Swords
Estimating the term…
The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in classical breeding, specific genetic elements are rearranged in vitro and inserted back into individual plant cells. Plants obtained through this new plant breeding process do not contain foreign nucleic acid but only contain nucleic acid from the plant species selected for transformation or plants that are sexually compatible with the selected plant species. Plants developed through this new plant breeding process are provided. In particular, potato plants displaying improved tuber storage and health characteristics are provided.
A method of modifying a trait of a plant, comprising (a) using Agrobacterium-mediated transformation to stably transform plant cells with a desired polynucleotide, wherein (i) the desired polynucleotide is located within a P-DNA or T-DNA of a vector, and (ii) the vector comprises a cytokinin integration marker gene in its backbone positioned outside of the P-DNA or T-DNA, wherein expression of the integration marker gene alters a morphological feature of a shoot that is regenerated from the transformed cells, (b) regenerating shoots from the cells, (c) identifying shoots, or plants obtained therefrom, that (i) do not have an altered morphological feature compared to shoots or plants regenerated from non-transformed cells, but (ii) do have the desired polynucleotide integrated into their genomes, wherein expression of the desired polynucleotide modifies a trait in the shoot or plant.
A transformation vector, comprising (i) a P-DNA or T-DNA, and (ii) a cytokinin gene positioned in the backbone of the vector outside of the P-DNA or T-DNA.
A method of transforming a plant cell with a desired polynucleotide, comprising (A) infecting a plant cell with an Agrobacterium strain carrying a vector that comprises (i) a desired polynucleotide located within a P-DNA or T-DNA, (ii) a selectable marker gene located in the P-DNA or T-DNA, and (iii) a cytokinin gene located in the backbone of the vector outside of the P-DNA or T-DNA;
A method of modifying a trait of a plant, comprising (A) using Agrobacterium-mediated transformation to stably transform plant cells with a desired polynucleotide positioned within a P-DNA of a vector, wherein the vector comprises an integration marker gene positioned in the vector backbone and outside of the P-DNA, (B) regenerating shoots from the cells, (C) identifying shoots, or plants obtained therefrom, that (i) do not have an altered morphological feature compared to shoots or plants regenerated from non-transformed cells, but (ii) do have the desired polynucleotide integrated into their genomes, wherein expression of the desired polynucleotide modifies a trait in the shoot or plant.
A method of modifying a trait of a selected plant, comprising:
Dependencies are inferred from the claim text.