Chimeric adenoviral vectors
AssigneeVaxart, Inc.
InventorSean N. Tucker
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Abstract
The present invention provides chimeric adenoviral vectors and methods for using the vectors to elicit an immune response to an antigen of interest.
Claims14
A chimeric adenoviral expression vector, said vector comprising an expression cassette comprising the following elements:
- (a) a first promoter operably linked to a nucleic acid encoding a toll-like receptor-3 (TLR-3) agonist, wherein the TLR-3 agonist is a double stranded RNA (dsRNA);
- and
- (b) a second promoter operably linked to a nucleic acid encoding a heterologous polypeptide, wherein said heterologous polypeptide is selected from the group consisting of:
- influenza virus HA, influenza virus Ml, influenza virus NP, HIV gag, and HIV env.
A chimeric adenoviral expression vector, said vector comprising an expression cassette comprising the following elements:
- (a) a first promoter operably linked to a nucleic acid encoding a toll-like receptor-3 (TLR-3) agonist, wherein the TLR-3 agonist is a double stranded RNA (dsRNA) and wherein the nucleic acid encoding the TLR-3 agonist comprises a sequence selected from the group consisting of:
- SEQ ID NOS:
- 8, 9, 10, 11, and 12;
- and
- (b) a second promoter operably linked to a nucleic acid encoding a heterologous polypeptide.
A method for eliciting an immune response, said method comprising administering to a mammalian subject an immunogenically effective amount of a chimeric adenoviral expression vector, said vector comprising an expression cassette comprising the following elements:
- (a) a first promoter operably linked to a nucleic acid encoding a toll-like receptor-3 (TLR-3) agonist, wherein the TLR-3 agonist is a double stranded RNA (dsRNA);
- and
- (b) a second promoter operably linked to a nucleic acid encoding a heterologous polypeptide,
- wherein the immune response is directed against the heterologous polypeptide, and wherein the route of administration is selected from the group consisting of:
- oral, intranasal, and mucosal.
Dependencies are inferred from the claim text.