US 7,862,724 B2Granted

Preparative liquid chromatograph system and preparative separation/purification method using the same

AssigneeShimadzu Corporation

InventorNaoki Osaka

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Abstract

A technique for extracting an objective component from a sample solution and obtaining the component in the form of equally separated powder aliquots is provided. A peak detector 12 detects the start point of the peak of the objective component on a chromatogram created from detection signals produced by a detector 5. Then, a controller 14 immediately changes a valve 6 so that the eluate will flow to a trap column 7a. A peak area processor 13 calculates the peak area of the objective component in real time. When the calculated area has exceeded a threshold, the controller 14 changes the valve 6 so that the eluate will flow to the next trap column 7b. Thus, the trap columns 7a to 7d are sequentially selected to divide the peak into one or more portions with the same area so that the objective component in the eluate will be sequentially captured by the trap columns 7a to 7d. Subsequently, the objective component is eluted from each trap column, and then the solvent is vaporized, to obtain powder of the objective component.

Claims8

Dependent claims

A preparative liquid chromatograph system having a liquid chromatograph including a column for separating a component from a separation sample and a detector for detecting the component contained in an eluate coming from the separation column, a plurality of trap columns for trapping the component contained in the eluate, and a channel selector for switching a channel so that the eluate is selectively supplied to one of the trap columns, comprising:

a) a chromatogram creator for creating a chromatogram during a chromatographic analysis of the sample, based on a detection signal produced by the detector;
b) a peak area calculator for performing a real-time calculation of an area value of a peak of an objective component appearing on the chromatogram, the calculation starting from a point in time where the channel is switched by the controller to be mentioned below;
and
c) a controller for controlling the channel selector so that a destination of the eluate coming from the column is changed from one trap column to another every time the area value calculated by the peak area calculator reaches a predetermined value.

1 more element

Dependencies are inferred from the claim text.