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US 7,858,388 B2Granted
MS based peptide and protein sequencing via reactions of lysine residues with peroxycarbonate compounds
AssigneeVanderbilt University
InventorsNed A. Porter, Douglas S. Masterson, Huiyong Yin, Richard M. Caprioli, Jeremy L. Norris
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Abstract
A method of modifying protein samples that comprises combining the sample with a peroxycarbonate solution and inserting the sample into a mass spectrometer. The present invention also includes methods of N-terminus characterization.
Claims9
A method of identifying protein or peptide sequences, comprising:
- providing a protein or peptide sample;
- providing a peroxycarbonate solution, wherein the peroxycarbonate solution comprises the following peroxycarbonate compound:
- wherein NO2 group is in para- or meta-position;
- combining the protein or peptide sample and the peroxycarbonate solution to perform a reaction between lysine residue amino groups and the peroxycarbonate compound which yields lysine peroxycarbamates;
- performing a mass spectrometric analysis of the lysine peroxycarbamates to obtain the protein or peptide sequence.
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Dependencies are inferred from the claim text.