Plasmid DNA isolation
AssigneeZymo Research Corporation
InventorXiyu Jia
Life of this patent
Estimating the term…
Abstract
Apparatus, reagents, and methods for isolating plasmid DNA from bacteria by alkaline lysis using a solid or immobilized P2 and/or P3 reagent in combination with a DNA-binding matrix.
Claims16
A lysate filtration device for filtering a bacterial alkaline lysate, the device comprising a mixing chamber, having a top and bottom opening, a P3 reagent provided in the mixing chamber as a dry formulation, a filtering media positioned in the mixing chamber, the filtering media being separate from the P3 reagent and in fluid contact with the mixing chamber, the mixing chamber being configured to receive a bacterial alkaline lysate through the top opening, wherein contact between the P3 reagent and the bacterial alkaline lysate can occur, whereby contact between the P3 reagent and the bacterial alkaline lysate will result in a neutralized lysate, the neutralized lysate further comprising a flocculent bacterial debris and a remaining bacterial lysate, and wherein the filtering media is for filtering the flocculent bacterial debris, from the remaining bacterial lysate.
A lysate filtration device for filtering a bacterial alkaline lysate, the device comprising a mixing chamber, having a top and bottom opening, a P2 and a P3 and reagent provided in the mixing chamber, the P2 and P3 reagents being disposed as dry formulations, wherein the P2 media is disposed to contact a cell suspension and dissolve before the P3 reagent, a filtering media positioned in the mixing chamber, the filtering media being separate from the P2 and P3 reagents, wherein the filtering media is in fluid contact with the mixing chamber, the mixing chamber being configured to receive a bacterial cell suspension through the top opening, whereby contact between the bacterial cell suspension and the P2 and P3 reagents can occur, and whereby contact between the bacterial cell suspension, the P2 reagent and the P3 reagent will result in a bacterial alkaline lysate that is rapidly neutralized, the neutralized lysate further comprising a flocculent bacterial debris and a remaining bacterial lysate, and wherein the filtering media is for filtering the flocculent bacterial debris, from the remaining bacterial lysate.
Dependencies are inferred from the claim text.