Polypeptide
AssigneeDanisco A/S
InventorsKarsten Matthias Kragh, Harm Mulder, Steffen Petersen, Helle Fomsgaard, Oene Robert Veltman
Life of this patent
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Abstract
We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.
Claims28
A non-naturally occurring PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises an alanine substitution at a position corresponding to position 223 of the Pseudomonas saccharophila exoamylase sequence of SEQ ID NO:
- 1, wherein the PS4 variant polypeptide has at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 1 and non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions, and which has a higher pH stability compared to the parent polypeptide when tested under the same conditions.
A non-naturally occurring PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, wherein said PS4 variant polypeptide has non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions, and which has a higher pH stability compared to the parent polypeptide when tested under the same conditions, and wherein said PS4 variant polypeptide is a polypeptide (a) which comprises all of SEQ ID NO:
- 1 except that it has alanine at a position corresponding to position 223 of SEQ ID NO:
- 1, or (b) which has alanine at a position corresponding to position 223 of SEQ ID NO:
- 1 and has at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 1.
A non-naturally occurring PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, wherein the PS4 variant polypeptide has (a) alanine at a position corresponding to position 223 of SEQ ID NO:
- 1, or (b) proline at a position corresponding to position 141 of SEQ ID NO:
- 1, wherein the PS4 variant polypeptide has at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 1 and non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions, and which has a higher pH stability compared to the parent polypeptide when tested under the same conditions.
A non-naturally occurring PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide has (a) alanine at a position corresponding to position 223 of SEQ ID NO:
- 1, (b) proline at a position corresponding to position 141 of SEQ ID NO:
- 1, or (c) alanine and proline at positions corresponding to position 223 and 141 of SEQ ID NO:
- 1, respectively, wherein the PS4 variant polypeptide has at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 1 and non-maltogenic exoamylase activity and a higher thermostability compared to the parent polypeptide when tested under the same conditions, and which has a higher pH stability compared to the parent polypeptide when tested under the same conditions.
A non-naturally occurring polypeptide having at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 1 in which the starch binding domain has been removed and wherein said polypeptide has (a) alanine at a position corresponding to position 223 of SEQ ID NO:
- 1, (b) proline at a position corresponding to position 141 of SEQ ID NO:
- 1, or (c) alanine and proline at positions corresponding to position 223 and 141 of SEQ ID NO:
- 1, respectively, wherein the polypeptide has non-maltogenic exoamylase activity.
A non-naturally occurring polypeptide having at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 4 in which the starch binding domain has been removed and wherein the said polypeptide has alanine at a position corresponding to position 223 of SEQ ID NO:
- 1, wherein the polypeptide has non-maltogenic exoamylase activity.
A non-naturally occurring polypeptide having at least 95% sequence identity with the polypeptide of SEQ ID NO:
- 3 in which the starch binding domain has been removed and wherein the said polypeptide has proline at a position corresponding to position 141 of SEQ ID NO:
- 1, wherein the polypeptide has non-maltogenic exoamylase activity.
Dependencies are inferred from the claim text.