Microorganisms for the production of 1,4-butanediol
AssigneeGenomatica, Inc.
InventorsMark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Jun Sun
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Abstract
The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO) pathway comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO. The invention additionally provides methods of using such microbial organisms to produce BDO.
Claims12
A host microbial organism transformed with nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, said microbial organism comprising exogenous nucleic acids encoding BDO pathway enzymes expressed in a sufficient amount to produce BDO, said BDO pathway enzymes comprising:
- a) 3-hydroxybutyryl-CoA dehydrogenase, said enzyme classified as EC 1.1.1.a and converting acetoacetyl-coenzyme A (CoA) to 3-hydroxybutyryl-CoA;
- b) 3-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting 3-hydroxybutyryl-CoA to crotonoyl-CoA;
- c) vinylacetyl-CoA A-isomerase, said enzyme classified as EC 5.3.3.3 and converting crotonoyl-CoA to vinylacetyl-CoA;
- d) 4-hydroxybutyryl-CoA dehydratase, said enzyme classified as EC 4.2.1.a and converting vinylacetyl-CoA to 4-hydroxybutyryl-CoA;
- and e) 4-hydroxybutyryl-CoA reductase (alcohol forming), said enzyme classified as EC 1.1.1.c and converting 4-hydroxybutyryl-CoA to 1,4-butanediol;
- or 4-hydroxybutyryl-CoA reductase, said enzyme classified as EC 1.2.1.b and converting 4-hydroxybutyryl-CoA to 4-hydroxybutanal, and 1,4-butanediol dehydrogenase, said enzyme classified as EC 1.1.1.a and converting 4-hydroxybutanal to 1,4-butanediol.
Dependencies are inferred from the claim text.