Method of combing a nucleic acid

A method of applying a nucleic acid to a surface, comprising:

• applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor;
• and
• controllably flowing a liquid deposition fluid over the first substrate by applying a cover surface to the deposition fluid in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of at least one of the cover surface and the first substrate.

A method of applying a nucleic acid to a surface, comprising:

• applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor;
• and
• controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises inducing voracity into the deposition fluid.

A method of applying a nucleic acid to a surface, comprising:

• applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor;
• and
• controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by applying a varying surface tension along the first substrate.

A method of applying a nucleic acid to a surface, comprising:

• applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor;
• and
• controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises directing fluid flow by controlling hydrophilicity of the first substrate.

A method of applying a nucleic acid to a surface, comprising:

• applying a nucleic acid-containing solution to a first substrate, the first substrate having a defined local physical feature spatially fixed thereon, the defined local physical feature comprising a nucleic acid anchor;
• and
• controllably flowing a liquid deposition fluid over the first substrate in a manner that promotes stretching of a nucleic acid molecule from the defined local physical feature along the first substrate, wherein controllably flowing comprises varying the temperature of the deposition fluid.