Mutant forms of meningococcal ADP-ribosylating toxin
AssigneeNovartis AC
InventorsMariagrazia Pizza, Vega Masignani
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Abstract
NMB1343 is an ADP-ribosylating toxin from Neisseria meningitidis. The invention provides a mutant toxin having a substitution at one or more of amino Glu-109, Glu-111 or Glu-120. The mutations(s) is/are preferably Glu to Asp. The protein of the invention preferably has reduced or eliminated ADP-ribosyltransferase and/or NAD-glycohydrolase activity relative to the wild-type protein.
Claims14
A polypeptide having at least 90% sequence identity to SEQ ID NO:
- 1, wherein the polypeptide has a substitution at one or more of amino acids corresponding to Glu-109, Glu-111 or Glu-120 of SEQ ID NO:
- 1.
A polypeptide comprising a fragment of a polypeptide having at least 90% sequence identity to SEQ ID NO:
- 1, wherein (i) the fragment includes one or more of amino acids corresponding to Glu-109, Glu-111 or Glu-120 from said polypeptide, and (ii) one or more of said amino acids corresponding to Glu-109, Glu-111 or Glu-120 is substituted.
The polypeptide of claim 6 or claim 7, wherein the fragment comprises at least 7 consecutive amino acids from the polypeptide.
Dependencies are inferred from the claim text.