Artificial immune system: methods for making and use

An in vitro two culture cellular system comprising:

• a vaccination site (VS) culture comprising a first substantially planar matrix, a plurality of cells consisting of endothelial cells and/or epithelial cells attached to said first matrix and an exogenous antigen of interest, for receiving a population of peripheral blood mononuclear cells (PBMCs) and for maturing dendritic cell precursors present in said population of PBMCs;
• and
• a three-dimensional artificial lymphoid tissue equivalent (LTE) culture comprising a second matrix and a plurality of lymphocytes attached to said second matrix, for receiving dendritic cells matured in said VS culture.