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US 7,851,675 B2Granted
Stress resistant plants
AssigneeBayer BioScience N.V.
InventorsMarc De Block, Michael Metzlaff, Veronique Gossele
Life of this patent
Estimating the term…
Abstract
Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.
Claims14
A method for obtaining a plant with increased stress resistance comprising
- a. introducing a chimeric gene into cells of a plant to obtain transgenic cells, said chimeric gene comprising the following operably linked DNA fragments:
- i. a plant-expressible promoter;
- ii. a DNA region coding for a nicotinamidase comprising the amino acid sequence of SEQ ID No.:
- 2;
- iii. a 3′ end region involved in transcription termination and polyadenylation;
- b. regenerating said transgenic cells to obtain a population of transgenic plants;
- and
- c. selecting a plant from said population of transgenic plants which exhibits increased stress resistance, exhibits a reduced level of reactive oxygen species, or maintains a high level of NADH under stress conditions when compared to a similar non-transgenic plant.
5 more elements
A chimeric gene comprising the following operably linked DNA fragments:
- (i) a plant-expressible promoter;
- (ii) a DNA region coding for a nicotinamidase comprising the amino acid sequence of SEQ ID No.:
- 2;
- (iii) a 3′ end region involved in transcription termination and polyadenylation.
1 more element
Dependencies are inferred from the claim text.