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US 7,851,188 B2Granted
Fermentive production of four carbon alcohols
AssigneeButamax(Tm) Advanced Biofuels LLC
InventorsGail K. Donaldson, Andrew C. Eliot, Dennis Flint, Lori Ann Maggio-Hall, Vasantha Nagarajan
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Abstract
Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.
Claims37
A recombinant microbial host cell comprising heterologous DNA molecules encoding polypeptides that catalyze substrate to product conversions for each step below:
- i) pyruvate to acetolactate;
- ii) acetolactate to 2,3-dihydroxyisovalerate;
- iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate;
- and
- iv) α-ketoisovalerate to isobutyraldehyde;
- wherein said microbial host cell produces isobutanol;
- and wherein
- a) the polypeptide that catalyzes a substrate to product conversion of pyruvate to acetolactate is acetolactate synthase having the EC number 2.2.1.6;
- b) the polypeptide that catalyzes a substrate to product conversion of acetolactate to 2,3-dihydroxyisovalerate is acetohydroxy acid isomeroreductase having the EC number 1.1.1.86;
- c) the polypeptide that catalyzes a substrate to product conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate is acetohydroxy acid dehydratase having the EC number 4.2.1.9;
- d) the polypeptide that catalyzes a substrate to product conversion of α-ketoisovalerate to isobutyraldehyde is branched-chain α-keto acid decarboxylase having the EC number 4.1.1.72.
8 more elements
Dependencies are inferred from the claim text.