Biological indicators for validating a prion sterilization-process
AssigneesPolyvalor S.E.C., Valorisation-Recherche
InventorsPierre Belhumeur, Karine Julien, Maryam Tabrizian, L'Hocine Yahia, Richard Marchand
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Abstract
The present invention relates to a method of evaluating the efficiency of sterilization processes by measurement of degradation level of prion protein indicators. When exposed to sterilization conditions, prion indicators are degraded in a manner to proportionally indicate the level of degradation of prion proteins themselves on medical devices or other surfaces usable in surgery and health cares.
Claims13
A method of evaluating the efficiency of a sterilization process on yeast prion proteins comprising:
- a) subjecting a sufficient amount of at least one prion protein degradation indicator in a container to said sterilization process such that degradation of prion proteins occurs;
- and
- b) determining the level of degradation of said prion protein degradation indicator,
- wherein said prion protein degradation indicator is transcribed by a gene selected from the group consisting of SUP35, URE2 and HET-s and is in amyloid form and wherein said level of degradation of said prion protein degradation indicator is indicative of the efficiency of said sterilization process.
A method of evaluating the efficiency of a sterilization process on yeast prion proteins, comprising:
- a) subjecting a sufficient amount of at least one prion protein degradation indicator in a container to said sterilization process such that degradation of the prion protein occurs, and
- b) determining the level of degradation of said prion protein degradation indicator,
- wherein said prion protein degradation indicator is selected from the group consisting of Sup35p, Ure2p, Het-s protein in amyloid form, and combination thereof and wherein said level of degradation of said prion protein degradation indicator is indicative of the efficiency of said sterilization process.
Dependencies are inferred from the claim text.