Human fetal bladder-derived epithelial cells
AssigneeRaven Biotechnologies, Inc.
InventorsRonghao Li, Zhuangyu Pan
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Abstract
This invention discloses a substantially pure population of human urinary bladder-derived epithelial cells and methods of isolating and culturing the urinary bladder-derived epithelial cells. In addition, several uses of human urinary bladder-derived epithelial cells and cells differentiating therefrom are disclosed herein.
Claims5
A method of isolating a substantially pure population of human fetal urinary bladder epithelial cells having a capacity to differentiate into either differentiated bladder or prostate epithelium, wherein said cells are positive for molecular marker H19 imprinted gene mRNA and lacking alpha-actin expression, comprising:
- (a) microdissecting human fetal urinary bladder tissue at gestational age from 14-21 weeks;
- (b) placing the microdissected human fetal urinary bladder tissue in a serum-free nutrient medium under culture conditions sufficient to sustain fetal urinary bladder epithelial cells wherein the serum-free medium contains nutrients comprising insulin, transferrin, α-tocopherol, and aprotinin;
- (c) maintaining suitable culture conditions sufficient to allow the migration of fetal urinary bladder epithelial cells from the human fetal urinary bladder tissue into the serum-free nutrient medium;
- and
- (d) maintaining suitable culture conditions to obtain a substantially pure population of fetal urinary bladder epithelial cells having a capacity to differentiate into differentiated bladder or prostate epithelium.
A substantially pure isolated population of human fetal urinary bladder epithelial cells having a capacity to differentiate into either differentiated bladder or prostate epithelium, wherein said cells are positive for molecular marker H19 imprinted gene mRNA and lacking alpha-actin expression, produced by the process comprising:
- (a) microdissecting human fetal urinary bladder tissue at gestational age from 14-21 weeks;
- (b) placing the microdissected human fetal urinary bladder tissue in a serum-free nutrient medium under culture conditions sufficient to sustain fetal urinary bladder epithelial cells wherein the serum-free medium contains nutrients comprising insulin, transferrin, α-tocopherol, and aprotinin;
- (c) maintaining suitable culture conditions sufficient to allow the migration of fetal urinary bladder epithelial cells from the human fetal urinary bladder tissue into the serum-free nutrient medium;
- and
- (d) maintaining suitable culture conditions to obtain a substantially pure population of urinary bladder epithelial cells.
A substantially pure isolated population of human fetal urinary bladder epithelial cells established from human fetal bladder tissue at gestational age from 14-21 weeks wherein said cells have a capacity to differentiate into either differentiated bladder or prostate epithelium and wherein said cells are positive for molecular marker H19 imprinted gene mRNA and lacking alpha-actin expression.
Dependencies are inferred from the claim text.