Back to results
US 7,846,697 B2Granted
Method of producing a low molecular weight organic compound in a cell
AssigneeBLMaps
InventorsJoergen Hansen, Thomas Hvid Andersen, Finn Thyge Okkels
Life of this patent
Estimating the term…
Abstract
A method of producing a low molecular weight organic compound (e.g. a plant or bacteria secondary metabolite) in increased yields involving use of a microorganism cell, which comprises a gene involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound and a glycosyltransferase gene capable of glycosylating the produced aglycon.
Claims7
A method of producing a low molecular weight organic aglycon compound comprising following steps:
- a) fermenting a microorganism cell in a suitable medium where the microorganism is capable of growing, the microorganism comprising:
- a gene encoding a product involved in the biosynthesis pathway leading to a low molecular weight organic aglycon compound;
- and,
- a glycosyltransferase gene encoding a glycosyltransferase capable of glycosylating the produced aglycon;
- wherein the cell produces the aglycon, the glycolsyltransferase and, by interaction of gylcosyltransferase with aglycon, the cell also produces the corresponding glycosylated form of the aglycon;
- b) deglycosylating the glycosylated form of the aglycon;
- and
- c) recovering the aglycon compound;
- (i) wherein the low molecular weight organic aglycon compound has a molecular weight from 50 to 3000, and (ii) wherein the glycosyltransferase is a glycosyltransferase capable of conjugating a sugar to the aglycon compound;
- and
- wherein the microorganism cell is a yeast cell;
- and
- wherein the low molecular weight organic aglycon compound is vanillin.
10 more elements
Dependencies are inferred from the claim text.