Herbicide resistance gene, compositions and methods
AssigneeThe Board of Trustees of the University of Illinois
InventorsPatrick Tranel, Aaron Hager, William Patzoldt
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Abstract
The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic and genetically engineered plant cells, plant tissue, seeds and plants which contain and express an herbicide resistant protoporphyrinogen oxidase such that they germinate from seed and grow in the presence of an amount of herbicide where the parent plant does not. Such plants are especially appropriate for use in agriculture or horticulture where herbicides are used to kill undesirable plants which might contaminate or compete with the transgenic plant of interest.
Claims13
A method for producing a genetically engineered, non-transgenic, herbicide resistant or tolerant plant cell, said method comprising the steps of:
- (a) introducing into a plant cell a recombinagenic oligonucleotide with a targeted mutation in the protoporphyrin oxidase (PPO) gene to produce plant cells with a mutant PPO gene that expresses a mutant PPO protein that is deleted at an amino acid position corresponding to Gly210 or Gly 211 in the Amaranthus tuberculatus PPO protein or at an analogous amino acid residue in a PPO homolog;
- and
- (b) identifying a plant cell having a mutant PPO protein that has substantially the same catalytic activity as compared to a corresponding wild type PPO protein in the presence of herbicide except that said mutant PPO protein is not inhibited by the herbicide;
- and
- whereby a non-transgenic herbicide resistant plant cell is produced.
A method of controlling weeds comprising the step of planting a non-transgenic, genetically engineered, herbicide resistant plant, plant, progeny plant or seed containing within its genetic complement the mutant PPO gene that expresses a mutant PPO protein that is deleted at an amino acid position corresponding to Gly210 or Gly 211 in the Amaranthus tuberculatus PPO protein or at an analogous amino acid residue in a PPO homolog;
- and treating the plants with a herbicide to which resistance is conferred by said molecule, whereby growth of weeds is controlled.
A method of selecting a non-transgenic, genetically engineered, herbicide resistant plant cell or plant tissue containing within its genetic complement the mutant PPO gene that expresses a mutant PPO protein that is deleted at an amino acid position corresponding to Gly210 or Gly 211 in the Amaranthus tuberculatus PPO protein or at an analogous amino acid residue in a PPO homolog, said method comprising the steps of growing said plant cell or plant tissue on a medium comprising an amount of an inhibitor of protoporphoryinogen oxidase sufficient to prevent growth of a corresponding plant cell which does not contain and express said nucleic acid molecule.
Dependencies are inferred from the claim text.