Fluorogenic probes for reactive oxygen species

A pro-fluorescent compound having the formula:

• wherein
• A and E are members independently selected from substituted or unsubstituted aryl wherein said substituted aryl is substituted with a group selected from halogen, R′, ═O, —OR′, ═NR′, —NR′R″, —NR″C(O)R′, —CONR′R″, —CO2R′ and —OC(O)R′, wherein R′ and R″ are independently selected from H and (C1-C8) alkyl;
• X is O;
• Z is a member selected from CR5, C(O), NR5 and substituted or unsubstituted heterocycloalkyl
• wherein
• R5 is a member selected from H, unsubstituted alkyl, and substituted or unsubstituted aryl, wherein said substituted aryl is substituted with a group selected from halogen, R′, ═O, —OR′, ═NR′, —NR′R″, —NR″C(O)R′, —CONR′R″, —CO2R′, —OC(O)R′, and a linker covalently attached to a member selected from a reactive functional group and a carrier selected from the group consisting of synthetic polymers and biomolecules, wherein R′ and R″ are independently selected from H and (C1-C8) alkyl;
• and
• said substituted heterocycloalkyl is substituted with a group selected from halogen, ═O, —OR′, ═NR′, —NR′R″, —NR″C(O)R′, —CONR′R″, —CO2R′, —OC(O)R′, and a linker covalently attached to a member selected from a reactive functional group and a carrier selected from the group consisting of synthetic polymers and biomolecules, wherein R′ and R″ are independently selected from H and unsubstituted alkyl;
• y is an integer selected from 0 and 1;
• q and r are members independently selected from 1, 2 and 3;
• and
• R1, R1′, R2, R2′, R3, R3′, R4 and R4′ are members independently selected from H,
• unsubstituted alkyl, and unsubstituted heteroalkyl.