Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library. application to the detection of mycobacteria

A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from:

• (a) nucleotide 1,696,728 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (b) nucleotide 1,698,096 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (c) nucleotide 1,700,210 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (d) nucleotide 1,701,293 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (e) nucleotide 1,703,072 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (f) nucleotide 1,704,091 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (g) nucleotide 1,705,056 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (h) nucleotide 1,705,808 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (i) nucleotide 1,706,631 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• or
• (j) nucleotide 1,707,530 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome.

A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from:

• (a) nucleotide 1,696,019 through nucleotide 1,698,096 of the Mycobacterium tuberculosis chromosome;
• (b) nucleotide 1,696,019 through nucleotide 1,700,210 of the Mycobacterium tuberculosis chromosome;
• (c) nucleotide 1,696,019 through nucleotide 1,701,293 of the Mycobacterium tuberculosis chromosome;
• (d) nucleotide 1,696,019 through nucleotide 1,703,072 of the Mycobacterium tuberculosis chromosome;
• (e) nucleotide 1,696,019 through nucleotide 1,704,091 of the Mycobacterium tuberculosis chromosome;
• (f) nucleotide 1,696,019 through nucleotide 1,705,056 of the Mycobacterium tuberculosis chromosome;
• (g) nucleotide 1,696,019 through nucleotide 1,705,808 of the Mycobacterium tuberculosis chromosome;
• (h) nucleotide 1,696,019 through nucleotide 1,706,631 of the Mycobacterium tuberculosis chromosome;
• or
• (i) nucleotide 1,696,019 through nucleotide 1,707,530 of the Mycobacterium tuberculosis chromosome.

A purified polypeptide, encoded by a polynucleotide, comprising an Open Reading Frame contained within SEQ ID NO:1, wherein the polynucleotide is selected from:

• (a) nucleotide 1,696,441 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (b) nucleotide 1,697,420 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (c) nucleotide 1,699,892 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (d) nucleotide 1,701,088 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (e) nucleotide 1,702,588 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (f) nucleotide 1,704,091 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (g) nucleotide 1,705,056 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (h) nucleotide 1,705,784 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• (i) nucleotide 1,707,524 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome;
• or
• (j) nucleotide 1,706,593 through nucleotide 1,708,746 of the Mycobacterium tuberculosis chromosome.