Nano-structured device for analysis or separation, and its preparation and application

A nano-structured support for analysis or/and separation, said nano-structured support comprising a nano-structured region,

• said nano-structured region comprising a solid phase support and a nano-structure fixed on said solid phase support,
• said nano-structure comprising non-aligned nano-structure units,
• said nano-structure units including nano-convex portions with a height over 3 nm, which cross-section at its half-height presents at least one dimension as 1-500 nm,
• wherein said nano-structured region has:
• 1) a distribution density of said nano-convex portions of more than 10 convexes/μm2;
• 2) a sensibility-increasing rate of more than 200%;
• and
• 3) any one or more of the following characteristics:
• (1) a nano-structure covering rate of more than 30%;
• (2) a surface-increasing rate of more than 400%;
• (3) an adsorption-increasing rate of more than 150%;
• and
• (4) an adsorption-attenuating rate of less than 80%,
• wherein said analysis or/and separation includes polypeptide or/and polypeptide-related-drug analysis or/and separation,
• wherein said nano-structure includes:
• (1) an inorganic nano-particle at least including:
• silica nano-particle or alumina oxide nano-particle;
• and/or
• 2) an organic nano-particle;
• and/or
• 3) a derivative of the inorganic and/or the organic nano-particle, which includes a derivative of inorganic and/or organic nano-particle which has bound chemical surface group and/or a coated organic compound;
• wherein said bound chemical surface group includes one or more of following chemical surface groups selected from the group consisting of amino-, aldehyde-, epoxy-, amino diazane, diethylaminoethyl, diethyl- (2-hydroxyproptyl) aminoethyl, carboxymethyl, sulfopropyl, mercaptoethylpyridine, siloxanyl, thioalcohol- and alkyl-;
• wherein said coated organic compound includes one or more of the following substances:
• (1) a surfactant including polyvinylpyrrolidone and/or nonionic surfactant;
• (2) a polyelectrolyte including polyamino acid;
• (3) an oleophilic compound including polysiloxane;
• (4) an ion exchange polymer including dextran derivative, agarose derivative, cellulose derivative, and/or polyacrylamide;
• or
• (5) affinity materials including heparin natrium, antigen and/or antibody;
• wherein said nano-structure units are in the shape of a tree branch
• and wherein said nanostructure support is prepared by the steps comprising
• 1) contacting a suspension of nano-particles with said support;
• and
• 2) fixing said nano-particles on said support,
• wherein said suspension has a concentration of from 1/20000 to 1/60000 g/ml in nano-particle weight/volume concentration.