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US 7,842,503 B2Granted
Hybrid recombinases for genome manipulation
AssigneePoetic Genetics, LLC
InventorMichele P. Calos
Life of this patent
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Abstract
The present invention provides methods to site-specifically manipulate genomes by using hybrid recombinases. Hybrid recombinases comprise a modified catalytic domain from a unidirectional serine phage integrase, fused to a foreign DNA recognition domain.
Claims12
A hybrid recombinase comprising:
- a catalytic domain having a catalytic activity of a first recombinase derived from a phiC31 or phiBT1 bacteriophage organism;
- and
- a DNA binding domain having a DNA binding activity of a second recombinase derived from a phiC31 or phiBT1 bacteriophage organism, wherein said first recombinase and said second recombinase are different, and wherein said hybrid recombinase has said catalytic activity of said first recombinase, and said DNA binding activity of said second recombinase.
Dependencies are inferred from the claim text.