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US 7,842,499 B2Granted
Substrates, devices, and methods for cellular assays
AssigneePlatypus Technologies, LLC
InventorsChristopher Murphy, Barbara Israel, Nicholas Abbott
Life of this patent
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Abstract
The present invention relates to the field of molecular diagnostics. In particular, the present invention provided improved substrates and methods of using liquid crystals and other biophotonically based assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal or other biophotonically based assay formats.
Claims18
A device for seeding cells in a well in a multiwell plate comprising:
- an insert sized to be inserted into a well of a multiwell plate,
- said insert having a first end and a second end and having at least one channel therein extending from said first end to said second end, said second end having an opening in fluid communication with said channel and comprising a solid projection extending from said second end, said solid projection having a perimeter smaller than the perimeter of said insert so that said channel opening is outside of said solid projection;
- wherein when said device is inserted into a well of a multiwell plate, said solid projection extending from said second end contacts the bottom of said well to seal off a portion of the bottom of said well and said opening provides fluid access to the bottom of said well wherein cells can be delivered to the bottom of said well via said at least one channel.
A system, comprising
- a multiwell plate;
- at least one insert sized to be inserted into a well of a multiwell plate,
- said insert having a first end and a second end and having at least one channel therein extending from said first end to said second end, said second end having an opening in fluid communication with said channel and comprising a solid projection extending from said second end, said solid projection having a perimeter smaller than the perimeter of said insert so that said channel opening is outside of said projection;
- wherein when said insert is inserted into a well of a multiwell plate, said solid projection extending from said second end contacts the bottom of said well to seal off a portion of the bottom of said well and said opening provides fluid access to the bottom of said well wherein cells can be delivered to the bottom of said well via said at least one channel.
1 more element
Dependencies are inferred from the claim text.