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US 7,842,496 B2Granted
Advanced selective plating media
AssigneeUniversity of Maryland
InventorsRussell G. Miller, Edward T. Mallinson
Life of this patent
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Abstract
Selective growth media N4 agars that include a combination of high levels of yeast extract, possibly higher levels of protein, elevated levels of sugar and reduced levels of sodium chloride. The combination of ingredients provides the ability to detect Salmonella spp. and Shigella spp. with almost identical sensitivity while alleviating false-negative and false-positive problems commonly encountered with the presence of Proteus spp. and Citrobacter spp.
Claims6
A non-chromogenic growth medium selective for Salmonella or Shigella comprising:
- yeast extract in an amount of about 7.0 g/l to about 30.0 g/l;
- a protein source;
- one or more carbohydrate sources in amounts effective to divert Citrobacter spp. from utilizing said protein source, and said carbohydrates sources being present in total amounts between about 18-65 g/l;
- and
- 7-ethyl-2-methyl-4-undecanol hydrogen sulfate.
2 more elements
Dependencies are inferred from the claim text.