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US 7,842,494 B2Granted
Micro-organisms for the treatment of soil and process for obtaining them
AssigneeAgro.Bio Hungary KFT.
InventorsGyorgy Botond Kiss, Istvan Ott
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AssigneeAgro.Bio Hungary KFT.
InventorsGyorgy Botond Kiss, Istvan Ott
Estimating the term…
The present invention relates to product(s) containing living microorganism(s) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as procedures for the production of the products, and procedures for the treatment of the soil and plants with the products. More particularly, the invention relates to a procedure for preparing the products from any of the microorganisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the microorganisms to be used. The subject invention also pertains to the microorganisms themselves. More particularly, the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the microorganisms selected from Azospirillum brasilense ssp. SW51 (NCAIM /P/ B 001293), Azotobacter vinelandii ssp. M657 (NCAIM /P/B 001292), Pseudomonas fluorescens var. SW11 (NCAIM /P/ B 001296), Bacillus polymyxa var. SW17 (NCAIM /P/ B 001295), Bacillus megaterium var. M326 (NCAIM /P/ B 001291), Micrococcus roseus ssp. A21 (NCAIM /P/B 001294), Bradyrhizobium japonicum var. PH25 (NCAIM /P/ B 001302), and Streptomyces albus var. 0003 LP (NCAIM /P/ B 001301), and furthermore the products multiplying and existing in the environment of the plant in question, containing the listed microorganisms and their production.
A preparation suitable for treatment of soil and plant seeds, which comprises a biologically pure culture of at least one living microorganism capable of propagating in different soil types in the environment of a plant, wherein one of said at least one microorganism is Bacillus megaterium var. M326 (deposited under NCAIM accession number NCAIM /P/ B001291) and said preparation comprises 5×106 to 5×1011 cells/gram of said preparation.
A process for preparing a preparation suitable for the treatment of soil and plant seeds, characterized by cultivating separately or together, in a culture medium containing a carbon source and a nitrogen source and inorganic salts, a biologically pure culture of at least one microorganism that can propagate at low temperature and in soils with low pH until reaching between 5×107 and 5×109 cells per milliliter, wherein one of said at least one microorganism is Bacillus megaterium var. M326 (deposited under NCAIM accession number NCAIM /P/ B001291).
A biologically pure culture of the microorganism Bacillus megaterium var. M326, deposited in the National Collection of the Agricultural and Industrial Microorganisms (NCAIM) under the depository accession number NCAIM /P/ B 001291.
A process for soil treatment comprising applying a preparation comprising a biologically pure culture of at least one microorganism wherein one of said at least one microorganism is Bacillus megaterium var. M326 (deposited under NCAIM accession number NCAIM /P/ B001291), in quantities of between 1010 and 1014 cells per hectare on or in the soil, in frost-free period.
A process for the treatment of plant seeds comprising treating the seeds with a liquid product comprising a biologically pure culture of at least one microorganism, wherein one of said at least one microorganism is Bacillus megaterium var. M326 (deposited under NCAIM accession number NCAIM /P/ B001291) and said liquid product comprises 5×106 to 5×1011 cells/gram of said liquid product.
Dependencies are inferred from the claim text.
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Bacteria; Culture media therefor: bacterial isolates
Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances…:…
Bacteria; Substances produced thereby or obtained therefrom: bacillus
Bacteria; Substances produced thereby or obtained therefrom: pseudomonas
About 8 months of term left · ends 9 Jul 2027 (estimated)
This application is the U.S. national stage application of International patent application No. PCT/HU02/00081, filed Aug. 12, 2002.
The present invention relates to product(s) containing alive micro-organism(s) suitable for soil treatment, micro-organisms multiplying under different climatic and natural circumstances, as well as the procedure for the production of the products, furthermore procedure for the treatment of the soil and plants with the products.
More detailed, the invention relates to a procedure for preparing the products from any of the micro-organisms specified below, or from the mixture thereof.
The Azospirillum species are the bacteria of Gram-negative-variable coloration, which are able to reduce the nitrogen of the air to ammonia under microaerofil circumstances (in the presence of 1-2% oxygen) and to make available these for the plants.
Azospirillum species were isolated from various soil samples (humous, loess, sodic, brown and black soil, etc.), from the root environment of various plants (cereals, sunflower, corn, grasses, etc.). The chemical attractants, such as the organic acids and sugars, attract the Azospirillum groups by chemotaxis. Helped by their flagella, the bacteria move towards the roots and having reached them, colonise.
From the 10-, 100-, 1.000- and 10.000-fold dilutions of the given soil sample made with sterile, distilled water, 100-100 μl suspension were spread on Petri-dishes containing MM and Nfb(II) soft agar. The composition of the MM medium is as follows: