Enhanced alcohol tolerant microorganism and methods of use thereof
AssigneeThe Regents of the University of Colorado
InventorsRyan T. Gill, Michael D. Lynch, Tanya Warnecke
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Abstract
Embodiments of the present invention provide methods and compositions for microorganisms having increased alcohol tolerance. In certain embodiments, methods for using such microorganisms, and methods for identifying gene or genetic regions responsible for increased alcohol tolerance are contemplated.
Claims13
A modified Escherichia coli (E. coli) microorganism that has been modified to increase the expression of a gene selected from the group consisting of yfdE, yhhL, yhhM, and a combination thereof, whereby an alcohol tolerance is enhanced, said modified microorganism thereby having increased alcohol tolerance relative to its wild type.
A method for producing ethanol comprising:
- culturing a modified Escherichia coli (E. coli) microorganism under conditions sufficient to produce ethanol, wherein the microorganism has enhanced expression of a gene selected from the group consisting of yfdE, yhhL, and yhhM, and a combination thereof;
- and recovering ethanol from the culture.
A method for enhancing alcohol resistance of a modified Escherichia coli (E. coli) microorganism comprising modifying the microorganism to increase expression of a gene selected from the group consisting of yfdE, yhhL, and yhhM, and a combination thereof.
Dependencies are inferred from the claim text.