Cytochrome c oxidase enzyme complex
AssigneeDSM Nutritional Products AG
InventorsAkira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Abstract
The present invention is directed to a novel cytochrome c oxidase complex, genetic materials useful for the preparation of the said complex, such as recombinant polypeptides involved in cytochrome c oxidase complex, recombinant DNA fragments, expression vectors, recombinant organisms and the like. Those novel cytochrome c oxidase complex and genetic materials may be originated from a microorganism having the identifying characteristics of Gluconobacter oxydans DSM 4025. The present invention also provides a method for the preparation of the said novel recombinant cytochrome c oxidase complex and a process for the production of 2-keto-L-gulonic acid (2KGA).
Claims8
A process for producing 2-keto-L-gluconic acid (2-KGA) from L-sorbose or D-sorbitol comprising:
- (a) cultivating, in a culture medium, a recombinant Gluconobacter oxydans host cell comprising the polynucleotide of SEQ ID NO:
- 1 or a polynucleotide that encodes the polypeptide of SEQ ID NO:
- 2;
- and
- (b) recovering 2-KGA from the culture medium.
A process for producing L-ascorbic acid from L-sorbose or D-sorbitol comprising:
- (a) producing 2-KGA by cultivating in a culture medium a recombinant Gluconobacter oxydans host cell comprising the polynucleotide of SEQ ID NO:
- 1 or a polynucleotide that encodes the polypeptide of SEQ ID NO:
- 2;
- (b) converting the 2-KGA into L-ascorbic acid;
- and
- (c) recovering L-ascorbic acid from the culture medium.
Dependencies are inferred from the claim text.