Methods for altering acetic acid production and enhancing cell death in bacteria
AssigneeBoard of Regents of the University of Nebraska
InventorsKenneth W. Bayles, Toni G. Patton, Kelly C. Rice
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Abstract
Methods of increasing cultured cell growth yields and/or protein production from bacterial cell cultures are provided. More particularly, mutant bacterial cells having an alteration in the expression or activity of the cidABC operon, a gene therein, or a homolog or a regulator thereof, and methods for reducing acetic acid/acetate production in cultures are provided, as are methods for increasing cultured cell growth yields and/or protein production employing such cells. Methods for enhancing bacterial cell death and methods for identifying agents that increase the susceptibility of bacteria to cell death are also provided.
Claims15
An isolated bacterial cell comprising an inactivating mutation in a Staphylococcus aureus cidC gene encoding the amino acid sequence set forth as SEQ ID NO:
- 2, which mutation measurably reduces acetate production by the cell when the cell is grown in medium comprising a metabolizable carbon source.
An isolated bacterial cell comprising an inactivating mutation in a gene encoding a Staphylococcus pyruvate oxidase polypeptide, wherein the pyruvate oxidase has at least 95% sequence identity with the amino acid sequence set forth as SEQ ID NO:
- 2, which inactivating mutation measurably reduces acetate production by the cell when the cell is grown in culture medium comprising a metabolizable carbon source.
An isolated bacterial cell comprising an inactivating mutation in a gene encoding a Staphylococcus pyruvate oxidase polypeptide, wherein the pyruvate oxidase has at least 98% sequence identity with the amino acid sequence set forth as SEQ ID NO:
- 2, which inactivating mutation measurably reduces acetate production by the cell when the cell is grown in culture medium comprising a metabolizable carbon source.
Dependencies are inferred from the claim text.