In vitro peptide expression library
AssigneeIsogenica Limited
InventorsDuncan McGregor, Richard Odegrip, Kevin Fizgerald, Rosemarie Hederer, Bill Eldridge, Chris Ullman, Philip Kuhlman, David Coomber
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Abstract
The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.
Claims34
A method for producing an in vitro peptide expression library comprising a plurality of peptides, wherein each peptide is non-covalently bound to the DNA construct encoding the peptide, comprising:
- (a) providing a DNA construct comprising:
- (i) a DNA target sequence;
- (ii) DNA encoding a library member peptide;
- and
- (iii) DNA encoding a peptide capable of non-covalently binding to said DNA target sequence of (a)(i);
- wherein said DNA construct and said peptide encoded by the DNA of (a) (iii) are selected to have cis-activity;
- and
- (b) expressing in an acellular environment a plurality of DNA constructs according to (a), wherein said DNA constructs encode a plurality of library member peptides such that each expressed peptide is non-covalently bound to the DNA from which it was produced.
Dependencies are inferred from the claim text.