Ultra sensitive biosensor for detecting bio-signal transmitters, and detector and detecting method using the same
AssigneeKorea Institute of Science and Technology
InventorsChangjoon Justin Lee, Dong-Ho Woo
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Abstract
The present invention relates to a novel use of a transfected cell as a biosensor, wherein the transfected cell expresses a receptor specific to a bio-signal transmitter, and a highly sensitive detector and a highly sensitive detecting method of a bio-signal transmitter using the biosensor. The bio-signal transmitter detecting technique according to the present invention has an advantage of being applied to a sniffer-patch method, and is capable of providing millisecond time resolution for detecting nanomolar levels of the bio-signal transmitter to be detected.
Claims5
A method of detecting a bio-signal transmitter comprising the steps of:
- preparing transfected cells expressing a receptor specific to the bio-signal transmitter to be detected and an ion channel regulated by the receptor;
- culturing cells isolated from a sample to prepare a cell monolayer;
- plating and co-culturing the transfected cells on the cell monolayer;
- and
- measuring current change in the co-cultured transfected cells through an electrode attached to cells, to detect the presence of the bio-signal transmitter from the cultured cells from the sample in real time,
- wherein the receptor is GluR1, GluR1LY, GluR2, GluR3, GluR4, GluR5, GluR6, GluR7, or mGluR1-7 and the bio-signal transmitter is glutamate, or the receptor is NR1-1a/NR2A2D and the bio-signal transmitter is D-serine or glycine.
Dependencies are inferred from the claim text.