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US 7,842,462 B2Granted
Identification of substances that inhibit NEMO oligomerization
AssigneeInstitut Pasteur
InventorsFrancois Traincard, Fabrice Agou, Michel Veron
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Abstract
The present invention provides methods for screening for substances which inhibit the oligomerization of NEMO and/or IKK-related complexes and/or signaling pathways based on the interference with NEMO oligomerization.
Claims11
A method of identifying a substance which inhibits IKK complex formation, comprising
- providing a cell deficient in endogenous NEMO and which is transformed with one or more polynucleotides that express at least two peptides that comprise a NEMO oligomerization region wherein the NEMO oligomerization region is at least one of coiled-coil motif or zinc finger moiety of the C-terminal domain of mammalian NEMO;
- applying the substance to the cell;
- and
- analyzing the level of IKK complex formation compared to the level of IKK complex formation in the absence of the substance, wherein a reduction of IKK complex formation in the presence of the substance indicates that the substance inhibits IKK complex formation.
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Dependencies are inferred from the claim text.