Method of producing analytical tool

A method of producing an analytical tool comprising a substrate made of polymethyl methacrylate having a keto group and formed with a groove for moving a sample liquid, and a cover bonded to the substrate to cover the groove,

• the method comprising:
• hydrophilically treating an inner surface of the groove of the substrate;
• and
• bonding the cover to the hydrophilized substrate;
• wherein the hydrophilization for the substrate includes a primary modifying step for bringing a modifying gas into contact with the inner surface of the groove to cause a primary substitution group from the modifying gas to bond to the keto group of the polymethyl methacrylate, and a secondary modifying step for substituting the primary substitution group bonded to the keto group with a hydroxyl group.