Electrophoresis
AssigneeWako Pure Chemical Industries, Ltd.
InventorsTomohisa Kawabata, Kenji Nakamura, Shinji Satomura
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Abstract
The invention relates to a method for separating a target for measurement utilizing electrophoresis, particularly capillary electrophoresis efficiently in high sensitivity and in a short period of time. It also relates to a method for measuring said target separated by said method for separation. The invention provides a method for separating a target for measurement and a method for measuring said target separated by said method for separation, characterized by using a substance to which is bound a nucleic acid chain labeled by a marker and which has an affinity for said target for measurement.
Claims34
A method for separation of a target for measurement which comprises using a complex-2 of i) a substance to which is bound a double-stranded nucleic acid chain and which has an affinity to the target and ii) a marker capable of labeling said double-stranded nucleic acid chain to form a complex-1 comprising a) the target, b) the substance and c) the marker, and separating said complex-1 from said complex-2 not involved in the formation of said complex-1 based on the difference of electrophoretic migration by electrophoresis.
A kit for measuring a target utilizing a separation based on the difference of electrophoretic migration by electrophoresis, comprising a substance to which is bound a double-stranded nucleic acid chain and which have an affinity for the target for measurement and a marker capable of labeling said double-stranded nucleic acid chain.
A kit for measuring a target utilizing a separation based on the difference of electrophoretic migration by electrophoresis, comprising a complex-2 of i) a substance to which is bound a double-stranded nucleic acid chain and which has affinity to the target for measurement and ii) a marker capable of labeling said double-stranded nucleic acid chain.
Dependencies are inferred from the claim text.