Holographic microscopy of holographically trapped three-dimensional structures
AssigneeNew York University
InventorsSang-Hyuk Lee, David G. Grier
Life of this patent
Estimating the term…
Abstract
A method and system for performing three-dimensional holographic microscopy of an optically trapped structure. The method and system use an inverted optical microscope, a laser source which generates a trapping laser beam wherein the laser beam is focused by an objective lens into a plurality of optical traps. The method and system also use a collimated laser at an imaging wavelength to illuminate the structure created by the optical traps. Imaging light scattered by the optically tapped structure forms holograms that are imaged by a video camera and analyzed by optical formalisms to determine light field to reconstruct 3-D images for analysis and evaluation.
Claims21
A method of performing 3-D holographic microscopy of an optically trapped structure, comprising the steps of:
- providing an optical microscope having a focal plane;
- generating a laser beam having an associated imaging wavelength input to the optical microscope wherein imaging of the optically trapped structure arises from the laser beam with the associated imaging wavelength;
- generating from the laser beam a plurality of optical traps with wave fronts of a trapping laser beam having a phase only hologram encoding an optical trapping pattern and the trapping laser beam having an associated trapping wavelength;
- and
- providing an objective lens for the optical microscope, the objective lens both projecting the plurality of optical traps and collecting in-line holographic images of the trapped structure;
- and
- providing volumetric images of the trapped structure from the laser beam to a device for providing 3-D holographic microscopy of the trapped structure.
A method of performing 3-D holographic microscopy of an object of an optically trapped structure, comprising the steps of:
- providing an optical microscope having a focal plane;
- generating a laser beam having an associated imaging wavelength input to the optical microscope wherein the imaging beam is provided by a laser having a beam output;
- providing an objective lens for the optical microscope, the objective lens at least collecting in-line holographic images of the object of the trapped structure;
- providing images of the object of the trapped structure to a device for viewing three-dimensional holographic microscopy;
- and
- analyzing the images using a Rayleigh-Sommerfeld formalism by analyzing propagation of the trapped structure along an optical axis of the optical microscope.
A system for performing three-dimensional holographic microscopy of an optically trapped structure, comprising:
- an optical microscope;
- a laser source for producing a trapping laser beam for a structure;
- a spatial light modulator for providing a phase-only hologram for imprinting on the trapping laser beam;
- the laser source also for producing an imaging laser beam and using the imaging laser beam providing volumetric three-dimensional images of the optically trapped structure;
- an objective lens associated with the optical microscope;
- a device for detecting laser light arising from imaging of the trapped structure and the device outputting image data;
- and
- a computer for analyzing the image data using computer software executed by the computer.
A system for performing three-dimensional holographic microscopy of an object of a structure, comprising:
- an optical microscope;
- a laser source for producing a collimated beam of coherent light for providing an imaging laser beam and selectively providing a trapping laser beam;
- a spatial light modulator for providing a phase-only hologram for imprinting on the trapping laser beam;
- an objective lens associated with the optical microscope for operating on the imaging laser beam and as needed selectively operating on the trapping laser beam;
- a device for detecting laser light arising from imaging of at least one of the object of the structure and selectively trapped features of the structure and the device outputting image data;
- and
- a computer for analyzing the image data using computer software executed by the computer.
Dependencies are inferred from the claim text.