Chloroplast transit peptides for efficient targeting of DMO and uses thereof
AssigneeMonsanto Technology LLC
InventorsPaul C. C. Feng, Marianne Malven, Stanislaw Flasinski
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Abstract
The invention provides for identification and use of certain chloroplast transit peptides for efficient processing and localization of dicamba monooxygenase (DMO) enzyme in transgenic plants. Methods for producing dicamba tolerant plants, methods for controlling weed growth, and methods for producing food, feed, and other products are also provided, as well as seed that confers tolerance to dicamba when it is applied pre- or post-emergence.
Claims33
A recombinant DNA molecule comprising a DNA sequence encoding a chloroplast transit peptide operably linked to a DNA sequence encoding dicamba monooxygenase, wherein the DNA sequence encoding the chloroplast transit peptide encodes SEQ ID NO:1.
A method for expressing dicamba monooxygenase in a plant cell comprising growing a plant comprising a nucleic acid construct comprising a nucleotide sequence encoding a pea Rubisco small subunit chloroplast transit peptide (CTP) of SEQ ID NO:1 operably linked to a nucleotide sequence encoding dicamba monooxygenase, thereby expressing the dicamba monooxygenase.
A dicamba tolerant seed comprising a DNA encoding a chloroplast transit peptide of SEQ ID NO:1 operably linked to a DNA encoding dicamba monooxygenase.
Dependencies are inferred from the claim text.