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US 7,838,222 B2Granted
Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
AssigneesThe United States of America/NIH, 20/20 GeneSystems, Inc.
InventorsVladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Abstract
The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
Claims25
A method for identifying one or more biomolecules from a plurality of wells in a multi-well plate comprising:
- providing a multi-well plate assembly;
- providing a stack of at least two layers;
- operatively securing the stack to the multi-well plate assembly;
- introducing samples comprising one or more biomolecules into two or more wells of the multi-well plate;
- transferring biomolecules from the wells to multiple layers in the stack;
- and
- identifying the one or more biomolecules on the multiple layers.
4 more elements
Dependencies are inferred from the claim text.