Back to results
US 7,829,303 B1Granted
Ligand screening method using a crystal of beta secretase
AssigneeElan Pharmaceuticals, Inc.
InventorsTimothy Earl Benson, Daisy Danielle Woods, Donald Bryan Prince
Life of this patent
Estimating the term…
Abstract
The x-ray crystal structure of BACE or BACE-like proteins is useful for solving the structure of other molecules or molecular complexes, and identifying and/or designing potential modifiers of BACE activity.
Claims9
A method of identifying the ability for a potential ligand to bind to Beta Amyloid Cleaving Enzyme (BACE), the method comprising:
- exposing a crystal of an unliganded BACE polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1, the crystal having space group symmetry C2221 and unit cell dimensions of a=75±5 Å, b=104±5 Å, c=100±5 Å, and α=β=γ=90°, to one or more samples comprising a potential ligand of BACE;
- and
- determining whether a ligand-BACE molecular complex is formed, wherein formation of a ligand-BACE molecular complex indicates the potential ligand has the ability to bind to BACE.
1 more element
A method of acquiring structural information for designing potential ligands for forming molecular complexes with BACE, the method comprising:
- exposing a crystal of an unliganded BACE polypeptide comprising the amino acid sequence of SEQ ID NO:
- 1, the crystal having space group symmetry C2221 and unit cell dimensions of a=75±5 Å, b=104±5 Å, c=100±5 Å, and α=β=γ=90°, to a library of potential ligands having diverse shapes;
- and
- determining whether a ligand-BACE molecular complex is formed, wherein formation of a ligand-BACE molecular complex indicates a potential ligand has a structure that forms a molecular complex with BACE.
1 more element
Dependencies are inferred from the claim text.