Device for reading plates bearing biological reaction support microdepositions
AssigneeInodiag
InventorsMichel Delaage, Gilles Nicolaï, Jean-Michel Decaudin, Pascal Huguet-Chantôme
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Abstract
A method for reading slides bearing fluorescent deposits, such as used in serology or molecular biology analysis, includes: incubating a slide with a sample of serum from a patient, or a dilution thereof, and revealing antibodies in the sample bound to the deposits by labelled reagents. Label reading and analysis is performed by a device including a slide positioner, light emitting diodes arranged in illumination channels to provide oblique illumination at different wave lengths, and a collection optic for forming an image of the deposits on a sensor.
Claims19
A method of serological analysis, comprising (a) incubating a serology slide comprising a reactive zone comprising a series of deposits of biological agents, with a sample of serum from a patient, or a dilution thereof, then (b) revealing antibodies in the sample bound to the deposits by labelled reagents, wherein label reading and analysis are carried out by a device comprising a means for positioning the slide, a means for illuminating the reactive zone and a collection optic, wherein:
- the means for illuminating the reactive zone comprises light emitting diodes arranged in illumination channels so as to enable an oblique illumination relative to an optical axis along which fluorescent light emitted by the microdeposits is captured by the collection optic;
- the device comprises at least two channels of diodes each emitting a specific excitation light;
- and
- the collection optic contains an objective forming an image of the deposits on a sensor.
Dependencies are inferred from the claim text.