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AssigneeBoard of Regents of the University of Nebraska
InventorsDonald P. Weeks, Xiao-Zhuo Wang, Patricia L. Herman
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The invention provides isolated and at least partially-purified dicamba-degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme(s) of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.
A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution.
A recombinant DNA molecule, consisting essentially of a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one addition.
A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution.
A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one addition.
A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence that differs from SEQ ID NO:3 by one substitution and by one addition.
A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence encodes an amino acid sequence comprising SEQ ID NO:3.
A plant transformation vector, comprising a DNA sequence encoding a dicamba-degrading oxygenase, wherein the DNA sequence comprises SEQ ID NO:2.
Dependencies are inferred from the claim text.