Wheat with altered branching enzyme activity and starch and starch containing products derived therefrom
AssigneesCommonwealth Scientific and Industrial Research Organization, Limagrain Cereales Ingredients S.A.
InventorsAhmed Regina, Sadequr Rahman, Matthew Kennedy Morell, Zhongyi Li
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Abstract
Wheat having a reduced level of SBEIIa activity, that may have a relative high amylose content. Wheat having a mutant SBEIIa gene in the A genome. The wheat might additionally have reduced levels of SBEIIb activity. The wheat grain of this invention can be of a non-shrunken phenotype despite a lesion in the amylopectin synthesis pathway, and may also have a high relative amylose content.
Claims32
A wheat grain comprising wheat endosperm having starch and DNA which encodes a double-stranded RNA molecule comprising nucleotides in a sequence which corresponds to a sequence of at least 30 contiguous nucleotides of an exon of the starch branching enzyme IIa (SBEIIa) gene whose sequence is set forth in SEQ ID NO:
- 1 and the complement thereof, which inhibits expression of SBEIIa, wherein the proportion of amylose of the starch of the wheat endosperm is at least 40% (w/w) as determined by an iodometric method.
A process of producing a wheat grain comprising wheat endosperm having starch, DNA which encodes a double-stranded hairpin RNA molecule comprising nucleotides in a sequence which corresponds to a sequence of at least 30 contiguous nucleotides of an exon of the starch branching enzyme IIa (SBEIIa) gene whose sequence is set forth in SEQ ID NO:
- 1 and the complement thereof, which inhibits expression of SBEIIa, and which wheat grain has a proportion of amylose of at least 40% (w/w) in the starch of the wheat endosperm as determined by an iodometric method, comprising the steps of growing a wheat plant that produces the wheat grain, and harvesting the wheat grain from the wheat plant.
Dependencies are inferred from the claim text.