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US 7,808,641 B2Granted
Interactive variable pathlength device
AssigneeC Technologies AB
InventorsMark Salerno, Tsung Shih, Craig Harrison
Life of this patent
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Abstract
This disclosure relates generally to a sampling device, and, more particularly, a sampling device that facilitates spectroscopic measurements with a variable path length and the necessary software controlled algorithms, and methods for using such a device.
Claims23
A method of determining the concentration of a sample comprising:
- (a) placing the sample solution in a vessel;
- (b) placing a probe within sample;
- (c) taking an absorbance reading;
- (d) moving the probe relative to the vessel with a unidirectional sliding mechanism by a predetermined increment such that a preselected path length through the solution is obtained such that a preselected path length through the solution is obtained and taking an absorbance reading at a predetermined wavelength;
- (e) repeating steps (c) and (d) sequentially one or more times;
- (f) generating a regression line from the absorbance values and path length such that a slope of the regression line is obtained;
- and,
- (g) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
5 more elements
A method of determining the concentration of a sample comprising:
- (a) placing the sample solution in a vessel;
- (b) moving a probe relative to the vessel such that the probe makes contact with the bottom of the vessel;
- (c) moving the probe relative to the vessel with a unidirectional sliding mechanism such that the probe moves from the bottom of the vessel through the sample by a predetermined increment such that a preselected path length through the solution is obtained;
- (d) taking an absorbance reading at a predetermined wavelength;
- (e) repeating steps (c) and (d) sequentially one or more times;
- (f) generating a regression line from the absorbance and path length such that a slope of the regression line is obtained;
- and,
- (g) determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
5 more elements
An instrument for determine the concentration of a sample at multiple path lengths comprising:
- (a) a light source operably linked to a probe;
- (b) a sample vessel that can contain the sample;
- (c) a motor operably linked to the sample vessel such that the sample vessel can be moved relative to the probe to provide variable path lengths;
- (d) a probe that can carry electromagnetic radiation that can be moved relative to the sample vessel by the motor;
- (e) a unidirectional sliding mechanism operably linked to the probe;
- (f) a detector located below the sample vessel that can detect electromagnetic radiation disposed such that the detector is substantially perpendicular to the electromagnetic radiation emanating from the probe and such that the electromagnetic radiation moves through the sample and is detected by the detector;
- and,
- (g) software for calculating the concentration of the sample based on the information provided by the detector at the predetermined path lengths;
- comprising generating a regression line from the absorbance and path length such that a slope of the regression line is obtained;
- and determining the concentration of the sample by dividing the slope of the regression line by the extinction coefficient of the sample.
7 more elements
Dependencies are inferred from the claim text.